（1）打开GUIDE，添加一个坐标轴并保存

（2）添加鼠标响应事件：鼠标按下事件，鼠标运动事件，鼠标松开事件

（3）对相应事件编写程序

function varargout = guide_m(varargin)
% GUIDE_M MATLAB code for guide_m.fig
%      GUIDE_M, by itself, creates a new GUIDE_M or raises the existing
%      singleton*.
%
%      H = GUIDE_M returns the handle to a new GUIDE_M or the handle to
%      the existing singleton*.
%
%      GUIDE_M('CALLBACK',hObject,eventData,handles,...) calls the local
%      function named CALLBACK in GUIDE_M.M with the given input arguments.
%
%      GUIDE_M('Property','Value',...) creates a new GUIDE_M or raises the
%      existing singleton*.  Starting from the left, property value pairs are
%      applied to the GUI before guide_m_OpeningFcn gets called.  An
%      unrecognized property name or invalid value makes property application
%      stop.  All inputs are passed to guide_m_OpeningFcn via varargin.
%
%      *See GUI Options on GUIDE's Tools menu.  Choose "GUI allows only one
%      instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES

% Edit the above text to modify the response to help guide_m

% Last Modified by GUIDE v2.5 25-Aug-2014 09:00:37

% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name',       mfilename, ...
                   'gui_Singleton',  gui_Singleton, ...
                   'gui_OpeningFcn', @guide_m_OpeningFcn, ...
                   'gui_OutputFcn',  @guide_m_OutputFcn, ...
                   'gui_LayoutFcn',  [] , ...
                   'gui_Callback',   []);
if nargin && ischar(varargin{1})
    gui_State.gui_Callback = str2func(varargin{1});
end

if nargout
    [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
    gui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT


% --- Executes just before guide_m is made visible.
function guide_m_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
% varargin   command line arguments to guide_m (see VARARGIN)

% Choose default command line output for guide_m
handles.output = hObject;

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%定义两个全局变量
global ButtonDown pos1;
ButtonDown =[];
pos1=[];
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

% Update handles structure
guidata(hObject, handles);

% UIWAIT makes guide_m wait for user response (see UIRESUME)
% uiwait(handles.figure1);


% --- Outputs from this function are returned to the command line.
function varargout = guide_m_OutputFcn(hObject, eventdata, handles) 
% varargout  cell array for returning output args (see VARARGOUT);
% hObject    handle to figure
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)

% Get default command line output from handles structure
varargout{1} = handles.output;


% --- Executes on mouse press over figure background, over a disabled or
% --- inactive control, or over an axes background.
function figure1_WindowButtonDownFcn(hObject, eventdata, handles)
% hObject    handle to figure1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%鼠标按下事件响应
global ButtonDown pos1;
if(strcmp(get(gcf,'SelectionType'),'normal'))%判断鼠标按下的类型，mormal为左键
    ButtonDown=1;
    pos1=get(handles.axes1,'CurrentPoint');%获取坐标轴上鼠标的位置
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

% --- Executes on mouse motion over figure - except title and menu.
function figure1_WindowButtonMotionFcn(hObject, eventdata, handles)
% hObject    handle to figure1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%鼠标运动事件的响应
global ButtonDown pos1;
if ButtonDown == 1
    pos = get(handles.axes1, 'CurrentPoint');%获取当前位置
    line([pos1(1, 1) pos(1, 1)], [pos1(1, 2) pos(1, 2)], 'LineWidth', 4);%划线
    pos1 = pos;%更新
end
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%


% --- Executes on mouse press over figure background, over a disabled or
% --- inactive control, or over an axes background.
function figure1_WindowButtonUpFcn(hObject, eventdata, handles)
% hObject    handle to figure1 (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%鼠标按键抬起的响应事件
global ButtonDown;
ButtonDown = 0;
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

（5）部分函数方法介绍

Matlab回调函数

SelectionType

Matlab学习-------GUI鼠标事件响应（鼠标划线实例）