cv::Mat img = cv::imread("image.jpg");

Method 1:

img.at<uchar>(i, j, k)

Method 2:

img.at<Vec3b>(i, j)[0]

Note: definitions for Vec

typedef Vec<uchar, 2> Vec2b;typedef Vec<uchar, 3> Vec3b;typedef Vec<uchar, 4> Vec4b;typedef Vec<short, 2> Vec2s;typedef Vec<short, 3> Vec3s;typedef Vec<short, 4> Vec4s;typedef Vec<int, 2> Vec2i;typedef Vec<int, 3> Vec3i;typedef Vec<int, 4> Vec4i;typedef Vec<float, 2> Vec2f;typedef Vec<float, 3> Vec3f;typedef Vec<float, 4> Vec4f;typedef Vec<float, 6> Vec6f;typedef Vec<double, 2> Vec2d;typedef Vec<double, 3> Vec3d;typedef Vec<double, 4> Vec4d;typedef Vec<double, 6> Vec6d;

OpenCV Code: Operations on Mat element